#crisp_bio注: 2019-10-15にタイトルのマイクロマニホールドパッチをマイクロニードルパッチに修正[出典] A luminal unfolding microneedle injector for oral delivery of macromolecules. Abramson A, Caffarel-Salvador E [..] Traverso G. Nat Med. 2019-10-07. [New ...
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2019年10月
xCas9のPAM拡張と高精度の構造基盤 (2)
[出典] Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease. Chen W [..] Gan J, Ji Q. PLoS Biol. 2019-10-11. Broad研のDavid R. Liuらは2018年に、野生型SpCas9 (WT-SpCas9)から定向進化システムPACE (phage-assisted continu ...
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マウス受精卵にRNPとssODNをエレクトロポレーション、7.67 Mbの染色体逆位とアレル特異的逆位/欠失誘導
“Technique for Animal Knockout system by Electroporation (TAKE)”; improved-Genome editing via Oviductal Nucleic Acids Delivery (i-GONAD); .... efficiently introduce megabases-sized chromosomal inversions and recessive lethal deletions ...
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BioIDを用いてHEK293細胞内タンパク質4,145種類の局在と35,902種類のPPIを同定
HUMAN CELL MAP - A BioID proximity map of the HEK293 proteome ...
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CRISPRメモ_2019/10/13 (7件)
HypaCas9 RNP delivery by nano some; HDR enhanced by MRN complex; SpCas9 PAM (5’-NGRNNNT-3’; SE/TFs for activation of quiescent Muscle Satellite Cells; breast cancer organoid model; gene editing in EndoC-βH1 cell; "FLASH" patent ...
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