1. [Keap1遺伝子ノックアウト実験] CRISPR/Cas9-Induced Loss of Keap1 Enhances Anti-oxidation in Rat Adipose-Derived Mesenchymal Stem Cells. Hu Y, Liu S, Zhu BM (四川大学). Front Neurol 2020-02-18
  2.  [Cas12a (Cpf1) & Cas9; 標的配列に依存してオンターゲットの10倍を超える編集が発生するオフタゲートサイトが存在] Specificity profiling of CRISPR system reveals greatly enhanced off-target gene editing. Wang Y [..] Du Q (北京大学). Sci Rep  2020-02-10
  3. [マウスにおける条件付きノックアウト・アレル作成法] Consistent Production of Mice with Conditional Knockout Alleles by CRISPR/Cas9-Mediated Genome Editing Using Two Guides/Two Oligos Approach. Golovko A et al. Cytology and Genetics 2020-01-18
  4. [細胞サーマル・シフト・アッセイによりCRISPR-Cas9ゲノム編集によるプロテオームの変化を捉える] Cellular thermal shift analysis for interrogation of CRISPR-assisted proteomic changes. Her NG [..] Nurmemmedov E. BioTechniques 2020-02-10
  5. [CRISPRシステムに対抗するファージ集団内の助け合い] [PREVIEW] In the Anti-CRISPR Jungle, Only the Weak Thrive? Croteau FR, Hynes AP. Cell Host & Microbe 2020-02-12; Exploitation of the Cooperative Behaviors of Anti-CRISPR Phages. Chevallereau A [..] Westra ER. Cell Host & Microbe 2020-02-12
  6. [オオタバコガで18種類のトリプシン遺伝子をCRISPR/Cas9でノックアウトしても、他のプロテアーゼ関連遺伝子の発現亢進を介して、プロテアーゼの総体としての活性は不変] Global gene expression changes induced by knockout of a protease gene cluster in Helicoverpa armigera with CRISPR/Cas9. Wang M [..] Wu Y. J Insect Physiology 2020-02-12
  7.  [Cas9に金ナノ粒子DNAプローブを融合し、リステリア・モノサイトゲネス, GMOおよびアフリカ豚コレラウイルスの超高感度検出を実現] CASLFA: Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay. Wang X [..] Zhou X. ACS Nano 2020-02-11.
  8. [深層学習によるsgRNA活性予測: 畳み込みニューラルネットワークと双方向ゲート付回帰型ユニット・ネットワークのハイブリッドと転移学習によるsgRNA活性予測; 入力はコード化したsgNRA配列と4種類のエピゲノムデータ] C-RNNCrispr: Prediction of CRISPR/Cas9 sgRNA activity using convolutional and recurrent neural networks. Zhang G, Dai Z, Dai X. Comput Struct Biotechnol J 2020-02-12
  9. [Cas12aのコラテラル活性を利用してエビ・サンプルから腸炎ビブリオの有無を紫外光照射を介して裸眼により5分で判定可能に] Selective endpoint visualized detection of Vibrio parahaemolyticus with CRISPR/Cas12a assisted PCR using thermal cycler for on-site application. Zhang M [..] Wu J, Chen H. Talanta 2020-02-11
  10. [非ウイルス性デリバリー法] Direct Cytosolic Delivery of Proteins Through Co-Engineering of Proteins and Polymeric Delivery Vehicles (PPNC). Lee YW [..] Rotello VM. J Am Chem Soc 2020-02-12
  11. [プロトコル] Genome Editing in the nematode Caenorhabditis briggsae using the CRISPR/Cas9 System. Culp E [..] Gupta BP. Biology Methods and Protocols 2020-02-10
  12. [プロトコル] Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9. Sinakevitch I, Kurtzman Z et alJoVE 2020-01-30
米国生物物理学会年会におけるCRISPR関連発表のタイトル - 6th Annual Meeting of the Biophysical Society (2020 Feb 15-19, San Diego)
  1. Biophysical Journal. Feb 07, 2020 Volume 118,Issue 3, Supplement 1, S1-626.
  2. Mapping the Boundary of DNA Unwinding in CRISPR-Cas9 Target Recognition
  3. Very Fast CRISPR on Demand
  4. Direct Observation of CRISPR-Cas12 Conformational Sampling by SM FRET and Cryo EM Reveals how Conformational Activation Promotes Catalysis and Resetting of the Endonuclease Activity
  5. Real-Time Observation of DNA Cleavage by CRISPR-Cas9 Endonuclease Using Pyrene Molecule as a Sensitive Probe for Detecting SUB-NM Structural Change
  6. Cardioprotection Conferred by a CRISPR/Cas9 Single Amino Acid Substitution of NCX1 (H165A): the PH Insensitive NCX Mouse
  7. Probing the RyR2 Ca2+ and Caffeine Binding Sites by Mutagenesis in Human Stem-Cell Derived Cardiomyocytes by CRISPR/Cas9 Gene Editing
  8. Crystal Structure of an Anti-CRISPR Protein, AcrIF2, and its Interaction With Type IF Cas Proteins
  9. Two-Metal Ion Mechanism of DNA Cleavage in CRISPR-Cas9
  10. A Catalytically Enhanced Type II-C Cas9 through Directed Protien Evolution