[注] 本稿はCRISP_SCIENCEのCRISPR関連2017年11月19日ツイートに準拠しています。

1. Class 2 type VI CRISPR-Cas関連特許
  • NOVEL CRISPR ENZYMES AND SYSTEMS. US 2017/0321198 A1. Pub. Date Nov. 9, 2017.
  • 出願人:THE BROAD INSTITUTE INC. (Cambridge, MA, US);  PRESIDENT AND FELLOWS OF HARVARD COLLEGE (Cambridge, MA, US); RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY (New Brunswick, NJ, US); SKOLKOVO INSTITUTE OF SCIENCE AND TECHNOLOGY (SKOLTECH) (Moscow, RU); THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERV (Bethesda, MD, US) 
  • 発明者:Severinov, Konstantin (Piscataway, NJ, US) ;Zhang, Feng (Cambridge, MA, US); Wolf, Yuri I. (Bethesda, MD, US); Shmakov, Sergey (Moscow, RU); Semenova, Ekaterina (Piscataway, NJ, US); Minakhin, Leonid (Piscataway, NJ, US);  Makarova, Kira S. (Bethesda, MD, US); Koonin, Eugene (Bethesda, MD, US); Konermann, Silvana (Zurich, CH);  Joung, Julia (Boston, MA, US);  Gootenberg, Jonathan S. (Cambridge, MA, US);  Abudayyeh, Omar O. (Boston, MA, US);  Lander, Eric S. (Cambridge, MA, US) 
  • [CRISPR_BIO 注]関連レビュー:Diversity and evolution of class 2 CRISPR–Cas systems. Shmakov S, Smargon A, Scott D, Cox D, Pyzocha N, Yan W, Abudayyeh OO, Gootenberg JS, Makarova KS, Wolf YI, Severinov K, Zhang F, Koonin EV. Nat Rev Microbiol. 2017 Mar;15(3):169-182. Epub 2017 Jan 23.
2. 'Minimal transportome'酵母プラットフォームの開発
  • トランスポート遺伝子25種類をノックアウトしたS. cerevisiae酵母株を作出し、Lactobacillus casei由来の乳酸脱水素酵素(LcLDH)の発現カセットを形質転換し、乳酸エクスポートの機序を探った。
  • A CRISPR/Cas9-based exploration into the elusive mechanism for lactate export in Saccharomyces cerevisiae. Mans R, Hassing EJ, Wijsman M, Giezekamp A, Pronk JT, Daran JM, van Maris AJA. FEMS Yeast Res. 2017 Nov 14.
3. CRISPR SAMによる転写活性化を利用して、アルツハイマー病モデル細胞システムを構築
4. ジフテリア毒素や緑膿菌外毒素Aの標的となるジフタミド生合成遺伝子の不活性化、ジフテリア毒素(DT)暴露およびピューロマイシン(PM)選択を組み合わせて、遺伝子編集効率の大規模測定を実現
  • 10,000-100,000個の細胞を対象として、1回の実験で遺伝子が標的が不活性化された数百のクローンを同定;DT耐性が二対立遺伝子ノックアウトの指標;PM耐性がカセット組み込みの指標;遺伝子不活性化には20塩基 gRNAが最適である一方カセット挿入には16-18塩基 gRNAが最適なことなどを同定
  • Disruption of diphthamide synthesis genes and resulting toxin resistance as a robust technology for quantifying and optimizing CRISPR/Cas9-mediated gene editing. Killian T, Dickopf S, Haas AK, Kirstenpfad C, Mayer K, Brinkmann U. Sci Rep 7, Article number: 15480DT and:or PM resistance
5. CRISPR-SpyCas9によるゲノム編集時のR-loop形成の動態を一分子FRET(smFRET)で解析
  • The initiation, propagation and dynamics of CRISPR-SpyCas9 R-loop complex. Zeng Y, Cui Y, Zhang Y, Zhang Y, Liang M, Chen H, Lan J, Song G, Lou J. Nucleic Acids Res. 2017 Nov 14.
Roop 1 Roop 2
6. ブタ内在レトロウイルス(PERV)不活性化が意味するところ
7. CRISPR/Cas9システムによるコリネ菌における遺伝子欠損、点変異生成および遺伝子挿入を実証
  • 遺伝子機能解析、代謝工学、標的生合成経路解析、組換えタンパク質発現などに有用
  • Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system. Peng F, Wang X, Sun Y, Dong G Yang Y, Liu X, Bai Z. Microb Cell Fact. 2017 Nov 14;16(1):201
8. CRISPR関連学会発表
  • American Heart Association's 2017 Scientific Sessions and Resuscitation Science Symposium Published in Circulation November 14, 2017, Volume 136, Issue Suppl 1.
  • Abstract 14957: CRISPR-mediated Expression of Fetal Scn5a Splice Variant Results in Arrhythmias and Conduction Defects
  • Abstract 15536: Crispr-mediated in vivo Confirmation of Rare Variants in Vinculin and Tropomysin1 Act Combinatorially to Dilated Cardiomyopathy
  • Abstract 15651: Inhibiting Immune Rejection and Enhancing Ischemic Hindlimb Repair by B2M Knockdown in Allogeneic Human MSC Using CRISPR/Cas9 Technology
  • Abstract 16195: Downregulation of B2M in Allogeneic MSC by CRISPR Technology Inhibits Immune Rejection and Enhances Cardiac Repair
  • Abstract 17178: CRISPR-mediated Insertions or Deletions of the Human LMNA Homolog in Zebrafish as a Model of Early-onset Cardiac Conduction Disease
  • Abstract 18021: Combining Human Induced Pluripotent Stem Cell Derived Cardiac Sheets, CRISPR-Based Genome Editing, and Optical Mapping of Rotors to Study the Short QT Syndrome
  • Abstract 18034: CRISPR/Cas9 Gene Editing Highlights the Prominent Role of Structure in Noncoding RNA Function: The Case of Clustered MicroRNAs in the Vasculature
  • Abstract 18717: Heart Repair by CRISPR-Induced Cardiovascular Progenitor Cells
  • Abstract 21011: Crispr/ndcas9 Adenoviral Vector System as a Tool for Promoting Mitochondrial Biogenesis in Cardiomyocytes
  • Abstract 21177: Seamless Gene Correction of LDLR in Familial Hypercholesterolemia Induced Pluripotent Stem Cells Mediated by CRISPR/Cas9 and PiggyBac