[出典] "Rational genome and metabolic engineering of Candida viswanathii by split CRISPR to produce hundred grams of dodecanedioic acid" Pham NN [..] Hu YC. Metab Eng 2023-03-20. https://doi.org/10.1016/j.ymben.2023.03.007[著者所属] National Tsing Hua U, ...
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タグ:合成生物学
部位特異的遺伝子導入の効率的な反復サイクルを介した非モデル菌・未培養菌に向けたハイスループット・ゲノム工学のツールを実現
[注] CRISPR-Cas技術に依存しないアプローチ[出典] "High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration" Elmore JR [..] Guss AM, Egbert RG. Sci Adv 2023-03-10. https://www.science.org/doi ...
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CRISPR/Cas9をベースとするサッカロマイセス・セレビシエ遺伝子座への多重遺伝子ノックイン法
[出典] "CMI: CRISPR/Cas9 Based Efficient Multiplexed Integration in Saccharomyces cerevisiae " Meng J [..] Shi S. ACS Synth Biol 2023-02-28. https://doi.org/10.1021/acssynbio.2c00591 [著者所属] Beijing U Chemical Technology, China Agricultural U, U Ill ...
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ShCASTをベースとする最適化トランスポゾンシステムとCRISPRiの組み合わせにより、大腸菌によるタンパク質生産を改善
[出典] "Metabolic engineering of Escherichia coli to enhance protein production by coupling ShCAST-based optimized transposon system and CRISPR interference" Chang CW [..] Hu YC. J Taiwan Inst Chem Eng. 2023-03-01. https://doi.org/10.1016/j.jtice.2023.10 ...
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枯草菌への一段階での複数遺伝子の同時組み込みを実現
[出典] "Barriers to simultaneous multilocus integration in Bacillus subtilis tumble down: development of a straightforward screening method for the colorimetric detection of one-step multiple gene insertion using the CRISPR-Cas9 system" Ferrando J, Fillue ...
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