タグ:可視化

[出典] "A Simple and Scalable Strategy for Analysis of Endogenous Protein Dynamics" Schwinn MK, Steffen LS, Zimmerman K, Wood KV, Machleidt T. Sci Rep 2020-06-02.  11アミノ酸のペプチドタグ (HiBiT)と~18 kDaのNanoLuc (LgBiT)と基質からなるHiBiTシステムを開 ... もっと読む

[出典] Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems.Yang LZ, Wang Y [..]  Chen LL. Mol Cell 2019 Dec 19;76(6): P981-997.E7 (On line Mol Cell 2019-11-9) [# 2020-01-17追記] [SPOTLIGHT] Put on Your Para-spectacles: The Development of Op ... もっと読む

[出典] Optimised insert design for improved single-molecule imaging and quantification through CRISPR-Cas9 mediated knock-in. Khan AO [..] Thomas SG, Morgan NV. Sci Rep. 2019-10-02. 英国Centre of Membrane and Protein and Receptorsを主とする研究グループは ... もっと読む

[出典] FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics. Kinjo T, Terai K [..] Matsuda M. Nat Methods 2019-09-09. 背景光誘導・光活性化二量体形成 (light-induced/light-activated dimerization; LID/LAD)技術の開発によって、 ... もっと読む

[出典] Live imaging of mRNA using RNA-stabilized fluorogenic proteins. Wu J, Zaccara S, Khuperkar D, Kim H, Tanenbaum ME, Jaffrey SR. Nat Methods. 2019 Sep;16(9):862-865. Online 2019-08-30.背景発蛍光性RNAアプタマー (以下、RNAアプタマー)によって標的mRNAの ... もっと読む

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