タグ:合成生物学

1. CRISPRツールキットを概観[出典] Commentary "The evolving CRISPR technology" Yan M, Li J. Protein Cell. 2019-06-26.Shanghai Institute of Biochemistry and Cell Biologyの研究チームが、拡張し続けるCRISPR Toolkitを概観 (Figure 1引用下図参照)2. [レビュー] CR ... もっと読む

[出典] “Conditional Guide RNAs: Programmable Conditional Regulation of CRISPR/Cas Function in Bacterial and Mammalian Cells via Dynamic RNA Nanotechnology” Hanewich-Hollatz MH [..] Pierce NA. ACS Cent Sci 2019-06-04.  [関連ハイライト] "Dynamic RNA Nano ... もっと読む

1. UC Irvine研究チーム、C末端タギングPITCh法をN末端タギングへと拡張[出典] "Microhomology based CRISPR tagging tools for protein tracking, purification, and depletion" Lin DW, Chung BP, Huang JW, Wang X, Huang L, Kaiser P. J Biol Chem 2019-05-28.C末端タギ ... もっと読む

[出典] "Total synthesis of Escherichia coli with a recoded genome" Fredens J, Wang K, de la Torre D, Funke LFH, Robertson WE [..] Chin JW. Nature 2019-05-15; (2019-05-18追記) [NEWS AND VEIEWS] "Construction of an Eshcherichia coli genome with fewer codon ... もっと読む

[出典] "Enabling large-scale genome editing by reducing DNA nicking" Smith CJ, Castanon O [..] Church GM. bioRxiv. 2019-03-15; V2 04-04. (# 2019-12-13 「塩基編集 (BE)により、一細胞内のLINE-1 26,100コピーのほぼ半数の同時不活性化を実現」から改題)概要HMS/Wys ... もっと読む

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