1. 多重なCRISPRアレイとcasクラスタの共存は、エピスタティックの相互作用を示唆する
[出典] "Co-occurrence of multiple CRISPRs and cas clusters suggests epistatic interactions" Bernheim A, Bikard D, Touchon M, Rocha E. bioRxiv. 2019-03-30.
- 2016年11月にNCBIゲノムデータベースから取得した2,268 バクテリアゲノムと167アーケアゲノム (4,453プラスミドゲノムとを含む；9,227プロファージを同定)、1,943ファージゲノムのCRISPRアレイとcasをin silicoで同定し、CRISPRアレイとcasの共存関係、CRISRシステムのタイプ・サブタイプの共存関係を解析；サブタイプの間にエピスタティックな関係 (ポジティブとネガティブの双方)が存在；プラスミドのCRISPRアレイによる宿主ゲノムのcas利用示唆；クラスタとファージとプロファージにも稀ではあるがCRISPR-Casシステムが存在
2. CRISPR-LbCas12aによる柑橘類ゲノム編集
[出典] "CRISPR-LbCas12a-mediated modification of citrus" Jia H, Orbović V, Wang N. Plant Biotechnol J. 2019-03-25.
- プロモーターとして35Sを利用することで、CRISPR-LbCas12aによるダンカン・グレープフルーツのゲノム編集を実現し、カンキツかいよう病菌に対する耐性付与
[出典] REVIEW "Applications of CRISPR Technologies Across the Food Supply Chain" Brandt K, Barrangou R. Annu Rev Food Sci Technol. 2019-03-25. 関連投稿 CRISPR-Directed Microbiome Manipulation across the Food Supply Chain" Barrangou R. Notebaart RA. Trends Microbiol. 2019-04-16.
- 食品産業に期待されている世界人口の膨張に見合うフードサプライチェーン拡充の手段の一つが、作物、家畜および微生物の品質改良である。これに答えるCRISPR-Cas技術の基礎と応用をNorth Carolina State UniversityのK. BrandtとR. Barrangouがレビュー
4. [特集号] 微生物CRISPR-Cas獲得免疫システムのエコロジーと進化 (レビューと論文のべ16編収録)
[出典] INTRODUCTION "The ecology and evolution of microbial CRISPR-Cas adaptive immune systems" Westra ER , van Houte S, Gandon S, Whitaker R. Philos Trans R Soc Lond B Biol Sci. 2019-03-25.
- 構成：CRISPR-Casシステムの進化と生物学的意味；自然環境におけるバクテリアとファージまたはアーケアとウイルスの共進化におけるCRISPR-Casシステムの役割；人工的な研究室環境における共進化のドライバーと結果；CRISPR-Casシステムの感染症抑止への応用と、その社会的・倫理的考察
5. [テキストブック] CRISPR遺伝子編集 - 手法とプロトコル
[出典] "CRISPR Gene Editing - Methods and Protocols" Luo Y. (eds) Part of the Methods in Molecular Biology book series
Methods for CRISPR-gRNA Design and Quantification of Activity
- CRISPR-gRNA Design: Maria Pallarès Masmitjà, Nastassia Knödlseder, Marc Güell
- Tracking CRISPR’s Footprints: Lin Lin, Yonglun Luo
- Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER: Eva Karina Brinkman, Bas van Steensel
- Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA: Saskia König, Zhang Yang, Hans Heugh Wandall, Claudio Mussolino, Eric Paul Bennett
- Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check: Lin Lin, Yonglun Luo
Methods for CRISPR Delivery
- CRISPR-Cas9 Delivery by Artificial Virus (RRPHC): Suleixin Yang, Qinjie Wu, Yuquan Wei, Changyang Gong
- Production and Validation of Lentiviral V箇条書きectors for CRISPR/Cas9 Delivery: Laura Barrett Ryø, Emil Aagaard Thomsen, Jacob Giehm Mikkelsen
- Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors: Julia Fakhiri, Manuela Nickl, Dirk Grimm
- Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs: Anders Laustsen, Rasmus O. Bak
CRISPR Gene Editing in Human iPSCs
- Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9: Saniye Yumlu, Sanum Bashir, Jürgen Stumm, Ralf Kühn
- Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes: Michaela Bruntraeger, Meg Byrne, Kathleen Long, Andrew R. Bassett
- Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9: Kirsten E. Snijders, James D. Cooper, Ludovic Vallier, Alessandro Bertero
CRISPR Gene Editing in Other Cell Types
- CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells: Daria Sergeeva, Jose Manuel Camacho-Zaragoza, Jae Seong Lee, Helene Faustrup Kildegaard
- Rapid and Efficient Gene Deletion by CRISPR/Cas9: Signe Neldeborg, Lin Lin, Magnus Stougaard, Yonglun Luo
- Genome Editing in Mice: Lisbeth Ahm Hansen, Ernst-Martin Füchtbauer
- CRISPR/Cas9-Mediated Gene Tagging: A Step-by-Step Protocol: Xi Xiang, Conghui Li, Xi Chen, Hongwei Dou, Yong Li, Xiuqing Zhang et al.
- Gene Editing in Primary Cells of Cattle and Pig: Petra Vochozkova, Kilian Simmet, Eva-Maria Jemiller, Annegret Wünsch, Nikolai Klymiuk
CRISPR Gene Therapy and Screening
- Toward In Vivo Gene Therapy Using CRISPR: Kristian Alsbjerg Skipper, Jacob Giehm Mikkelsen
- CRISPR Gene Therapy of the Eye: Targeted Knockout of Vegfa in Mouse Retina by Lentiviral Delivery: Andreas Holmgaard, Sidsel Alsing, Anne Louise Askou, Thomas J. Corydon
- In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection: Francesco Niola, Frederik Dagnæs-Hansen, Morten Frödin
- CRISPR-Based Lentiviral Knockout Libraries for Functional Genomic Screening and Identification of Phenotype-Related Genes: Emil Aagaard Thomsen, Jacob Giehm Mikkelsen
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