blogging about crisp bio-S&T, especially CRISPR.


1.SpCas9活性と遺伝子ドライブを抗-CRISPRタンパク質 (AcrIIA2; AcrIIA4)によって調節する:酵母での実証[出典] "Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae" Basgall EM, ~ Finnigan GC. Microbiology. 2018 Fe ... もっと読む

1.evoCas9:酵母in vivoスクリーン法を開発し、高い選択性を示すSpCas9変異体を同定[出典]"A highly specific SpCas9 variant is identified by in vivo screening in yeast" Casini A, ~ Cereseto A. Nat Biotechnol. 2018 Mar;36(3):265-271. Published online 2018 Jan ... もっと読む

出典"Atomic structures of low-complexity protein segments reveal kinked β sheets that assemble networks" Hughes MP, ~ Eisenberg, DS. Science. 2018 Feb 9;359(6376):698-701背景細胞には、P-body (日本語/英語)、パラスペックル (paraspeckle; 下図参照)およびス ... もっと読む

1.MhAX (microhomology assisted excision):マイクロホモロジー媒介末端結合 (MMEJ)を介したヒトiPSCsのスカーレス・ゲノム編集"Microhomology-assisted scarless genome editing in human iPSCs" Kim S, ~ Woltjen K. Nat Commun. 9, Article number: 939. 2018 Mar 5.MM ... もっと読む

出典"Bifunctional immune checkpoint-targeted antibody-ligand traps that simultaneously disable TGFβ enhance the efficacy of cancer immunotherapy" Ravi R, ~ Bedi A8. Nat Commun. 2018 Feb 21;9(1):741. Y-trapsの設計TGFβは腫瘍微小環境において、Tregsの誘導 ... もっと読む