blogging about crisp bio-S&T, especially CRISPR.


出典Targeting wild-type KRAS-amplified gastroesophageal cancer through combined MEK and SHP2 inhibition. Wong GS [..] Catenacci D, Bass AJ. Nat Med. 2018 May 28.背景KRAS遺伝子変異に起因するKARSタンパク質の変異は膵臓癌の97.7%、大腸癌の44.7%および肺癌の30.9 ... もっと読む

出典"A novel three-dimensional high-throughput screening approach identifies inducers of a mutant KRAS selective lethal phenotype". Kota S[..]Kissil J, Spicer Tp. Oncogene. 2018 May 10.背景RASタンパク質は膵臓癌、肺癌、および大腸癌など癌において最も高頻度 ... もっと読む

1.塩基エディターBE4とABEを、BE4max/AncBE4max/ABEmaxへと強化[出典] "Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction" Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP, Raguram A,  Liu ... もっと読む

1.[Research Highlight] CRISPR/Cas9が誘発する'scars' (indels)から細胞系譜を辿る[出典] "Tracking development with DNA ‘scars’" Kling J. Lab Animal 2018 May 23.8連続GFPに刻まれる'scars'をもとにゼブラフィッシュ発生時の細胞系譜追跡を実現したAlemany等の研 ... もっと読む

1.縦列重複の増幅 (コピー数増加)が、癌細胞必須遺伝子のCRISPR-Cas9ドロップアウトスクリーンに偽陽性をもたらす[出典] "Tandem duplications lead to loss of fitness effects in CRISPR-Cas9 data" Gonçalves E [..] Garnett MJ (Sanger Inst.). bioRxiv Posted May 25, ... もっと読む