blogging about crisp bio-S&T, especially CRISPR.


1. マウス胚において、sgRNAと3塩基ミスマッチのサイトで、高頻度なオフターゲット編集が発生[出典] "CRISPR/Cas9 can mediate high-efficiency off-target mutations in mice in vivo" Aryal NK, Wasylishen AR,  Lozano G. Cell Death & Diseases 2018 Oct 27.Dicer1を標 ... もっと読む

[出典] "CRISPR-Sirius:RNA scaffolds for signal amplification in genome imaging" Ma H,Tu LC, Naseri A, Chung YC, Grunwald D, Zhang S, Pederson T. Nat Methods. 2018 Oct 30. ("CRISPR-Based DNA Imaging in Living Cells Reveals Cell Cycle-Dependent Chromosome D ... もっと読む

[出典] "Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation" Liu LD, Huang M, Dai P [..]  Zhao Y, Yeap, LS, Meng  FL. Cell Rep. 2018 Oct 23.背景塩基編集 (BE)法は、BE3、YEE-BE3、SaBE4-GamおよびTarget-A ... もっと読む

1. [プロトコル] scGESTALT: トランスクリプトームとCRISPR-Cas9バーコードの単一細胞における読み出しにもとづく大規模な細胞系譜の再構成[出典] "Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPR–Cas9 barcodes by ... もっと読む

[出典] Research Highlight "SIRT6 at the beginning" Neff EP. Lab Animal. 2018 Oct 23.;NEWS AND VIEWS "Role for the longevity protein SIRT6 in primate development" Nature. 2018 Aug;560(7720):559-560.;論文 "SIRT6 deficiency results in developmental retard ... もっと読む