blogging about crisp bio-S&T, especially CRISPR.


[出典] "Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9" Hirano S [..] Nishimasu H, Nureki O. Nat Commun. 2019-04-29;構造情報 PDB 6JOO: Crystal structure of Corynebacterium diphtheriae Cas9 in complex with sgRNA a ... もっと読む

[出典] "Cholesterol Induces CD8+T Cell Exhaustion in the Tumor Microenvironment" Ma X, Bi E [..] Yi Q. Cell Metabolism. 2019-04-25.  腫瘍浸潤T細胞は高頻度で抗腫瘍性を失う。Houston Methodist Research Institute (以下、HMRI) を主とする研究グループは、腫瘍 ... もっと読む

[出典] "Novel screening system revealed that intracellular cholesterol trafficking can be a good target for colon cancer prevention" Miyamoto S [..] Mutoh M. Sci Rep. 2019-04-17.がん化学予防は、天然物、合成または生物学的製剤を利用して、発がんの初期または ... もっと読む

[出典] "Artificial sgRNAs engineered for genome editing with new Cas12b orthologs" Teng F, Cui T, Gao Q,  Guo L, Zhou Q, Li W. Cell Discov. 2019-04-23.概要State Key Laboratory of Stem Cell and Reproductive Biology (Beijing)の研究チームは今回、ヒト細胞ゲ ... もっと読む

1. 高精度版CRISPR-Cas9の活性を、tRNAGlnプロセシング・システムを利用して、ブーストする[出典] "Boosting activity of high-fidelity CRISPR/Cas9 variants using a tRNAGln-processing system in human cells" He X, [..] Gu F. J Biol Chem. 2019-04-22.オフターゲット ... もっと読む