[出典] "FLASH - a next-generation CRISPR diagnostic for multiplexed detection of antimicrobial resistance sequences" Quan J, Langelier C, Kuchta A [..] Crawford ED. Nucleic Acids Res 2019-05-22;特許公開 US20190300935A1 Method for finding low abundance …
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2019年05月
金コロイドナノ粒子が、Cas12a (Cpf1)-crRNAを造血幹細胞の核に届け、遺伝子治療技術を僻地に届ける
[出典] "Targeted homology-directed repair in blood stem and progenitor cells with CRISPR nanoformulations" Shahbazi R [..] Adair JE. Nat Mater 2019-05-27;"Special delivery: Gold nanoparticles ship CRISPR cargo" Siegel J. Fred Hutch News Service 2019-05-2 …
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Cas13は、ファージ感染細胞を休眠させることでDNAファージから守り、集団免疫にも貢献する
[出典] "Cas13-induced cellular dormancy prevents the rise of CRISPR-resistant bacteriophage" Meeske AJ, Nakandakari-Higa S, Marraffini LA. Nature 2019-05-29;NEWS AND VIEWS "Bacterial dormancy curbs phage epidemics" Jackson SA, Fineran PC. Nature 2019-05- …
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合成DNAを制限修飾系に対してステルス化し、バクテリアの形質転換効率を10万倍に
[出典] "Systematic evasion of the restriction-modification barrier in bacteria" Johnston CD, Cotton SL, Rittling SR, Starr JR, Borisy GG, Dewhirst FE, Lemon KP. PNAS 2019-05-16;"SyngenicDNA: stealth-based evasion of restriction-modification barriers duri …
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CRISPRメモ_2019/05/29 (6件)
1. Cas9が誘導する大規模なゲノム欠損において、マイクロホモロジーが高頻度でみられる[出典] "Microhomologies are prevalent at Cas9-induced larger deletions" Owens DDG [..] Teboul L, de Bruijn MFTR. Nucleic Acids Res 2019-05-25.University of OxfordとMRC Harwel …
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