blogging about crisp bio-S&T, especially CRISPR.


[出典] "Restore natural fertility of Kitw/Kitwv mouse with nonobstructive azoospermia through gene editing on SSCs mediated by CRISPR-Cas9" Li X, Sun T, Wang X, Tang J, Liu Y. Stem Cell Res Ther. 2019-08-24.広州医学院第一附属医院とInstitute of Zoology (北 ... もっと読む

[出典] "Recovery of the non-functional EGFP-assisted identification of mutants generated by CRISPR/Cas9" Ren C [..] Liang Z. Plant Cell Rep. 2019-08-24.  Institute of Botany (北京)の研究グループは今回、標的細胞からのDNA抽出・解析不要のスクリーン法を開発 ... もっと読む

[出典] ”Structural insight into multistage inhibition of CRISPR-Cas12a by AcrVA4” Peng R, Li Z, Xu Y [..] Shi Y, Gao GF.PNAS 2019-08-29.背景 Lachnospiraceae bacterium Cas12a (LbCas12a)によるヒト細胞におけるゲノム編集がanti-CRISPRタンパク質AcrVA4によっ ... もっと読む

[出典] "Anti-CRISPR-Associated Proteins Are Crucial Repressors of Anti-CRISPR Transcription" Stanley SY [..] Davidson AR. Cell 2019-08-29. ; [crisp_bio 2019-11-07追記] [in Brief] Anti-anti-CRISPR. Tang L. Nat Methods. 2019-10-31. 背景ファージはanti-CRISP ... もっと読む

[出典] "Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements" Satchwell TJ, Wright KE [..] Baum J. Nat Commun. 2019-08-23.U. Bristolと Imperial College Londonなどの研究グループは今回、不死化ヒト ... もっと読む