[出典] "CRISPR-Cas9-Mediated Intersectional Knockout of Glycogen Synthase Kinase 3β in D2 Receptor Expressing Medial Prefrontal Cortex Neurons Reveals Contributions to Emotional Regulation" Khlghatyan J, Beaulieu JM. CRISPR J 2020-06-17. セリンンスレオ …
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2020年06月
接合性プラスミドIncCが、Vibrio choleraeのCRISPR-Cas9による分解を回避する機構
[出典] "IncC conjugative plasmids and SXT/R391 elements repair double-strand breaks caused by CRISPR–Cas during conjugation" Roy D, Huguet KT, Grenier F, Burrus V. Nucleic Acids Res 2020-06-18. Université de Sherbrookeの研究チームは今回、コレラ菌のタ …
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CRISPR-Cas9 HDRを介した標識により、生細胞内での内在GPCRの動態観察を実現
[出典] "CRISPR-Mediated Protein Tagging with Nanoluciferase to Investigate Native Chemokine Receptor Function and Conformational Changes" White CW [..] Hill SJ. Cell Chem Biol 2020-05-21; PREVIEW "Playing Tag with Your Favorite GPCR Using CRISPR" Horioka …
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うどんこ病耐性トマト作出の観点からCRSIPR/Cas9に拠り感受性遺伝子PMR4に変異誘導
[出典] "CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew" Santillán Martínez MI [..] Bai Y. BMC Plant Biol 2020-06-19. Wageningen University & Researchの研究グループは今回、トマトのPMR4遺 …
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CRISPR転写活性化にタイプI-F Cascade-VPRはdCas9とdCas12a-VPRに優る
[出典] "Repurposing type I–F CRISPR–Cas system as a transcriptional activation tool in human cells" Chen Y, Liu J [..] Liang P, Songyang Z. Nat Commun 2020-06-19. クラス2 CRISPR-CasはCRISPR-Casシステムの~10%を占めるに過ぎないが、タイプ IIのCas9, タ …
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