blogging about crisp bio-S&T, especially CRISPR.


[出典] "Comparative CRISPR type III-based knockdown of essential genes in hyperthermophilic Sulfolobales and the evasion of lethal gene silencing" Zink IA [..] Schleper C. RNA Biology. 2020-09-21.  University of ViennaとUniversity College Londonの研究グ ... もっと読む

[出典] "ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems" Xie J, Huang X [..] Wang K, Lai L. BMC Biology. 2020-09-23.  CBEとABEが発表されて以来、失活させたCas9ヌクレアーゼの両端にそれぞれ、種々のアデノシンデ ... もっと読む

[出典] "Inhibition of DNA ligase IV enhances the CRISPR/Cas9-mediated knock-in efficiency in mouse brain neurons" Cao X, Kouyama-Suzuki E [..] Tabuchi K. Biochem Biophys Res Commun. 2020-09-21.  信州大学を主とする研究グループは今回、DNAリガーゼIV (LIG4) ... もっと読む

[出典] "Genome-wide CRISPR knockout screen identifies ZNF304 as a silencer of HIV transcription that promotes viral latency" Krasnopolsky S, Kuzmina A, Taube R. PLoS Pathog 2020-09-21.  Ben-Gurion University of the Negevの研究チームは今回、標題宿主因子 ... もっと読む

[出典] "CRISPR-based engineering of gene knockout cells by homology-directed insertion in polyploid Drosophila S2R+ cells" Xia B [..] Perrimon N. Nat Protoc. 2020-09-21.背景 CRISPR遺伝子ノックアウトには主として、ヌクレアーゼが誘導するDSBからのNHEJを介し ... もっと読む