blogging about crisp bio-S&T, especially CRISPR.


[出典] "CEN‐tools: an integrative platform to identify the contexts of essential genes" Sharma S, Dincer C, Weidemüller P, Wright GJ, Petsalaki E. Mol Syst Biol. 2020-10-19.  EMBLとWellcome Sanger Instituteの研究チームが今回、Context‐specific Essenti ... もっと読む

[出典] "Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination" Bashir S, Dang T, Rossius J, Wolf J, Kühn R. BMC Biotechnol. 2020-10-23.  Cas9-sgRNAとDSB修復用のDNAテンプレート (以下、ドナー)による相同組換修 ... もっと読む

[出典] "A cell cycle-dependent CRISPR-Cas9 activation system based on an anti-CRISPR protein shows improved genome editing accuracy" Matsumoto D, Tamamura H, Nomura W. Commun Biol. 2020-10-23; [BEHIND THE PAPER] Use of a clustered regularly interspaced s ... もっと読む

2020-10-27 更新: MRC-LMB, hermo Fisher Scientificなどによる成果について初稿はbioRxiv投稿に準拠していましたが、今回、査読付き論文としてNature誌から出版されたことから、その書誌情報を追記します - "Single-particle cryo-EM at atomic resolution" Nakane T, Ko ... もっと読む

[出典] "Conditional guide RNA through two intermediate hairpins for programmable CRISPR/Cas9 function: building regulatory connections between endogenous RNA expressions" Lin J, Liu Y, Lai P, Ye H, Xu L. Nucleic Acids Res. 2020-10-17.  中山大学 (Sun Yat ... もっと読む