[出典] "CrisPam: SNP-Derived PAM Analysis Tool for Allele-Specific Targeting of Genetic Variants Using CRISPR-Cas Systems" Rabinowitz R, Almog S, Darnell R, Offen D. Front Genet. 2020-08-18. https://doi.org/10.3389/fgene.2020.00851 Tel Aviv Universityの …
続きを読む
2020年11月
[レビュー] 1塩基分解能の実現: CRISPR-Cas遺伝子編集の特異性向上
[出典] REVIEW "Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing" Rabinowitz R, Offen D. Molecular Therapy 2020-11-26. https://doi.org/10.1016/j.ymthe.2020.11.009 Tel Aviv Universityの著者らは、オフターゲット活性 …
続きを読む
[プロトコル] CRISPR/Cas9によるヒト初代B細胞のゲノム工学
[出典] "Genome Engineering of Primary Human B Cells Using CRISPR/Cas9" Laoharawee K, Johnson MJ, Lahr WS, Peterson JJ, Webber BR, Moriarity BS. JoVE 2020-11-03. https://doi.org/10.3791/61855 PBMC(末梢血単核細胞)に由来するCD19陽性B細胞におけるCD19のノッ …
続きを読む
慢性B型肝炎ウイルス感染ヒト化マウスモデルのCRISPR/Cas9遺伝子編集
[出典] "CRISPR/Cas9 gene editing of hepatitis B virus in chronically infected humanized mice" Stone D [..] Jerome KR. Mol Ther-Meth Clin D. 2020-11-26. https://doi.org/10.1016/j.omtm.2020.11.014 Fred Hutchinson Cancer Research Center, University of Washi …
続きを読む
CBE/ABEとPEによる遺伝子編集結果の評価にはT7E1に替えてSIPATAを
[出典] "One-step genotyping method in CRISPR based on short inner primer-assisted, tetra primer-paired amplifications" Lee NE, Ha DI, Ko JH, Kim YS. Mol Cell Probes 2020-11-25. https://doi.org/10.1016/j.mcp.2020.101675 CRISPR-Casによるゲノム編集結果の評 …
続きを読む