[出典] "First Report of CRISPR/Cas9 Mediated DNA-Free Editing of 4CL and RVE7 Genes in Chickpea Protoplasts" Badhan S, Ball AS, Mantri N. Int J Mol Sci. 2020-12-30. https://doi.org/10.3390/ijms22010396 RMIT University (メルボルン)の研究チームが二種類の耐 ...
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2021年01月
リンゴとナシのCRISPR/Cas9ゲノム編集の限界を越える新戦略: キメラの回避とBE
[出典] "New Strategies to Overcome Present CRISPR/Cas9 Limitationsin Apple and Pear: Efficient Dechimerization and Base Editing" Malabarba J, Chevreau E [..] Vergne E. Int J Mol Sci. 2020-12-30. https://doi.org/10.3390/ijms22010319 University Angersを主 ...
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[レビュー] Cas9-sgRNA RNP複合体のデリバリー戦略
[出典] REVIEW "Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing" Zhang S, Shen J, Li D, Cheng Y. Theranostics. 2021-01-01. https://doi.org/10.7150/thno.47007 華南理工大学, 華東師範大学に台州市第二人民医院が加わった研究 ...
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[プロトコル] RNPデリバリーと選択マーカ用遺伝子編集を介した線虫ゲノム編集
[出典] PROTOCOL "CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes" Smith M, Bergwell M, Smith E, Mathew D, Iyer J. J Vis Exp 2020-12-11. https://doi.org/10.3791/62001 ...
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ヒト造血幹細胞・前駆細胞の遺伝子ノックアウト効率をsgRNAをレンチウイルスでCas9 mRNAをエレクトロポレーションでデリバリーすることで大きく向上
[出典] "Combined lentiviral- and RNA-mediated CRISPR/Cas9 delivery for efficient and traceable gene editing in human hematopoietic stem and progenitor cells" Yudovich D, Bäckström A [..] Larsson J. Sci Rep 2020-12-28. https://doi.org/10.1038/s41598-020- ...
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