[出典] "Identifying novel therapeutic targets in gastric cancer using genome-wide CRISPR-Cas9 screening" Zeng Z, Zhang X, Jiang C-Q [..] Tie X-Y, Jiang Y-A. Oncogene 2022-02-17. https://doi.org/10.1038/s41388-022-02177-1 武漢大学附属病院とOhio State Univ …
続きを読む
2022年02月
CRISPR/Cas9を介して,希少疾患に関連する意義不明の変異を帯びた遺伝子をHEK293細胞に導入し,トランスクリプトミクスを施すことで,その変異の意義を同定
[出典] "Functional validation of variants of unknown significance using CRISPR gene editing and transcriptomics: A Kleefstra syndrome case study" Fear VS et al. Gene 2922-02-18. https://doi.org/10.1016/j.gene.2022.146287 Perth Children’s HospitalとThe U …
続きを読む
CRISPR/Cas9とssODNを用いた実用的な両アレル遺伝子欠失戦略により,NPHP1ノックアウトヒト多能性幹細胞を作製
[出典] "Generation of NPHP1 knockout human pluripotent stem cells by a practical biallelic gene deletion strategy using CRISPR/Cas9 and ssODN" Nakano T, Susa K [..] Sohara E. In Vitro Cell Dev Biol Anim. 2022-02-14. https://doi.org/10.1007/s11626-022-0065 …
続きを読む
シングルセルCRSIPRスクリーン結果を擾乱するクローン細胞拡大を検証
[出典] "Computational identification of clonal cells in single-cell CRISPR screens" Wang Y, Xie S, Armendariz D, Hon GC. BMC Genomics. 2022-02-15. https://doi.org/10.1186/s12864-022-08359-1 University of Texas Southwestern Medical Centerの研究グループは …
続きを読む
CRISPR/Cas9による水痘帯状疱疹ウイルスの複製および神経細胞活性化の抑制
[出典] "Antiviral Targeting of Varicella Zoster Virus Replication and Neuronal Reactivation Using CRISPR/Cas9 Cleavage of the Duplicated Open Reading Frames 62/71" Wu BW [..] Kinchington PR. Viruses 2022-02-12. https://doi.org/10.3390/v14020378 Universit …
続きを読む