[注] PARP阻害剤 (PARPi)[出典] "CRISPR/Cas9‑induced saturated mutagenesis identifies Rad51 haplotype as a marker of PARP inhibitor sensitivity in breast cancer" Yang H [..] Ren L, Li X. Mol Med Rep 2022-06-17. https://doi.org/10.3892/mmr.2022.12774 [ …
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2022年06月
SaCas9によりマウス体内で編集したB細胞が, 高力価の抗HIV広域中和抗体を分泌
[出典] "In vivo engineered B cells secrete high titers of broadly neutralizing anti-HIV antibodies in mice" Nahmad AD [..] Barzel A. Nat Biotechnol 2022-06-09. https://doi.org/10.1038/s41587-022-01328-9 [著者所属] Tel Aviv U, Tabby Therapeutics Ltd, St …
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Cas9/sgRNA RNPのエレクトロポレーションにより,初代細胞での高レベル遺伝子編集を実現
[出典] "Electroporation-Mediated Delivery of Cas9 Ribonucleoproteins Results in High Levels of Gene Editing in Primary Hepatocytes" Rathbone T [..] Cottle RN. CRISPR J 2022-06-08/2022-03-02. https://doi.org/10.1089/crispr.2021.0134[著者所属] Clemson Unive …
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一塩基対の遺伝子編集に成功後の標的部位を, CRISPR-Cas9による再切断から保護するHideRNA
[出典] "HideRNAs protect against CRISPR-Cas9 re-cutting after successful single base-pair gene editing" Hermsen TJW [..] Te Riele H. Sci Rep 2022-06-10. https://doi.org/10.1038/s41598-022-13688-y [著者所属] The Netherlands Cancer Institute, Leiden U, U …
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ほぼPAM-lessなSpGとSpRYを利用することでゼブラフィッシュのゲノム編集の適用範囲を拡大
[出典] "SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish" Liang F [..] Liu Y, Qin W. Nat Commun 2022-06-14. https://doi.org/10.1038/s41467-022-31034-8. [著者所属] South China Normal U, Guangdong Provincial People's Hospita …
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