[出典] "In vivo base editing by a single intravenous vector injection for treatment of hemoglobinopathies" Li C [..] Lieber A. JCI Insight 2022-08-25. https://doi.org/10.1172/jci.insight.162939 [著者所属] University of Washington, Broad Institute, Fred …
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2022年09月
CRISPR/Cas9を利用して、3種類のTTNノックアウト・ヒトiPSCを樹立
[注] TTN (タイチン遺伝子)[出典] "Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system" Kang JY, Mun D, Chun Y [..] Lee SH, Joung B. Stem Cell Res 2022-08-27. https://doi.org/10.1016/j.scr.2022.102901 [著者所 …
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[プロトコル] 合成致死遺伝子ペアをのコンビナトリアルCRISPRスクリーニング用デュアルgRNAライブラリーの構築
[出典] PROTOCOL "Generation of dual-gRNA library for combinatorial CRISPR screening of synthetic lethal gene pairs" Tang S [..] Li L. STAR Protocols 2022-08-24. https://doi.org/10.1016/j.xpro.2022.101556 [著者所属] The Ohio State University, University of …
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CRISPR-Casの毒素-抗毒素RNAガードは、リピート配列の縮退を介して高い特異度を帯びている
[注] 毒素-抗毒素システム (toxin-antitoxin system, TA system < addiction module)[出典] "The toxin-antitoxin RNA guards of CRISPR-Cas evolved high specificity through repeat degeneration" Cheng F, Wu A, Liu C [..] Xiang H, Li M. Nucleic Acids Res 2022-08-26 …
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[レビュー] CRISPR-Casバイオセンサーによる新型コロナウイルス変異株検出におけるプライマーとガイドRNAの評価
[出典] REVIEW "In Silico Evaluation of CRISPR-Based Assays for Effective Detection of SARS-CoV-2" Kaewsapsak P, Chantaravisoot N, Nimsamer P, Mayuramart O, Mankhong S. Pathogens 2022-08-25. https://doi.org/10.3390/pathogens11090968 [著者所属] Chulalong …
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