[注] hiPSC (ヒトiPS細胞)[出典] "Generation of a homozygous and a heterozygous SNCA gene knockout human-induced pluripotent stem cell line by CRISPR/Cas9 mediated allele-specific tuning of SNCA expression" Schneider Y [..] Xiang W. Stem Cell Res 2022-10-20 ...
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2022年10月
タイプV CRISPR-Casシステムの中で、シトシン (C) リッチなPAMを認識する新たなCas12lファミリーを発見
[出典] "A new family of CRISPR-type V nucleases with C-rich PAM recognition" Urbaitis T, Gasiunas G, Young JK [..] Siksnys V. EMBO Rep 2022-10-21. https://doi.org/10.15252/embr.202255481 [著者所属] CasZyme, Vilnius U, Corteva Agriscience™ (コルテバ・アグ ...
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LbCas12aの標的可能範囲を拡大し、発がん遺伝子の多重編集を実現
[出典] "Expanded targeting scope of LbCas12a variants allows editing of multiple oncogenic mutations" Choi E, Hwang HY, Kwon E, Kim D, Too T. Mol Ther Nucleic Acids 2022-09-19. https://doi.org/10.1016/j.omtn.2022.09.005 [著者所属] Kyung Hee U, Sungkyunkwa ...
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酵母ゲノム工学のためのCRISPR-Casシステムのアセンブリーとin vivo編集を、最小限の試薬と時間で実現するACtivE法を開発
[出典] "ACtivE: Assembly and CRISPR-Targeted in Vivo Editing for Yeast Genome Engineering Using Minimum Reagents and Time" Malcı K [..] Rios-Solis L. ACS Synth Biol 2022-10-17. https://doi.org/10.1021/acssynbio.2c00175 [著者所属] U Edinburgh, Newcastle ...
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CRISPR/Cas9を利用してアレルのノックアウトとアホロートルを作出
[出典] "Applying a Knock-In Strategy to Create Reporter-Tagged Knockout Alleles in Axolotls" Wang L, Zeng YY, Liu Y, Wei JF. In: Seifert AW, Currie JD. (eds) Salamanders. MIMB 2022-10-23. https://doi.org/10.1007/978-1-0716-2659-7_23 [著者所属] South China ...
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