[出典] REVEIW "Development of Single Molecule Techniques for Sensing and Manipulation of CRISPR and Polymerase Enzymes" Chu J, Romero A, Taulbee J, Aran K. Small 2023-05-24. https://doi.org/10.1002/smll.202300328 [著者所属] Keck Graduate Institute, Cardea, UC Berkeley; 本文 18ページ/参考文献 217件

[構成]
1. Introduction
2 Optical Techniques
Optical Biosensing
2.1.1 Fluorescence Resonance Energy Transfer (FRET)
Optical Manipulation
2.2.1 Optical Tweezers (OTs)
2.2.2 Utilization of Optical Tweezers for Characterizing CRISPR Function
2.2.3 Utilization of Optical Tweezers for Characterizing Polymerase Function
2.2.4 Optical Tweezers Limitations and Prospects
3 Mechanical Techniques
Mechanical Biosensing
3.1.1 Atomic Force Microscopy (AFM)
Mechanical Manipulation
3.2.1 Single-Molecule Force Spectroscopy by AFM (AFM-SMFS)
3.2.2 Magnetic Tweezers (MTs)
4 Electronic Techniques
Electronic Biosensing
4.1.1 Nanowires
4.1.2 Field-Effect Transistors (FETs)
4.1.3 Nanowires and FET Limitations and Prospects
Electronic Manipulation
4.2.1 Nanowires
4.2.2 Nanopores
5 Other Techniques
Other Techniques Biosensing
5.1.1 Cryogenic Electron Microscopy (Cryo-EM)
6 Conclusion and Discussion