[出典] Review "Next-generation CRISPR/Cas-based ultrasensitive diagnostic tools: current progress and prospects" Sahel DK, Giriprasad G [..] Chitkara D. RSC Adv. 2024-10-14. https://doi.org/10.1039/D4RA04838E [所属] Birla Institute of Technology and Science (India), KK Birla Goa Campus
[crisp_bio注] これまでに公開されたアッセイ法を確認するのに便利
[crisp_bio注] これまでに公開されたアッセイ法を確認するのに便利
[構成]
Introduction
Biosensors
Nucleic acid as a biomarker in diagnosis
CRISPR/Cas systems
Fig. 1 Evolutionary timeline of the CRISPR.
CRISPR/Cas in nucleic acid diagnostics
Fig. 2 Physiochemical properties of different Cas effector proteins.
Workflow of CRISPR-based biosensors
Fig. 3 Overview of the basic mechanism involved while adopting CRISPR as biosensors
CRISPR-based biosensors: extensive evidence
Table 1 Different nucleic acid detection methods using CRISPR/Cas effector proteins
Fig. 4 Schematic illustration of key applications of CRISPR-based biosensors.
CRISPRD
RPA-CRISPR-Cas12a
CRISPR/Cas12a system with a digital microfluidic chip (DMF)
iSCAN
iSCAN-V2
Fig. 5 The iSCAN-V2 assay with Cas12b can efficiently detect SARS-CoV-2.
CRISPR-eSPR
FELUDA
Fig. 6 FELUDA for SARS-CoV-2 detection.
CREST
SATORI
CASCADE
PPCas12 assay
Ultralocalized Cas13a assay
TwistDx
SHINE
STOP-Covid
PECL-CRISPR
CARMEN
APC-Cas
E-CRISPR
HOLMES
HOLMESv2
DETECTR
SHERLOCK/CRISPR-Dx
SHERLOCKv2
Harnessing nanotechnology to improve feasibility of CRISPR diagnostics
Table 2 Different types of nanoparticle carriers used in CRISPR diagnostics
Conclusions and future prospects
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