天津医学大学の機能イメージング重点研究室に籍を置く研究者達を主とする中国の研究チームが今回、CRISPR-Cas9 GE（ゲノム編集）の精密な時空間制御の戦略として、化学分子を介した制御と対比させて、優れた時空間制御の精度、可逆性、生体適合性を備えている外部からの物理的刺激による制御を対象とするレビューを発表した。以下に、両者の利点と弱点の対比表（出典のTable 1）と後者の概観（出典のFigure ２）を引用し、レビューの構成を紹介する：
[構成] 参考文献196件を含む27頁
1. Introduction
2. Physical stimuli-responsive CRISPR-Cas9 systems with spatiotemporal precision
2.1 Optical control
2.1.1 Optical control of (d/n)Cas9
2.1.2 Optical control of sgRNA function
2.1.3 Optical control of non-viral delivery systems
2.2 Thermal control
2.2.1 Temperature-sensitive Cas9 variants or inhibitors
2.2.2 Heat-inducible promoter-mediated control
2.2.3 Biomaterials-mediated thermal control
2.3 Magnetic control
2.3.1 Magnetic field-guided delivery
2.3.2 Magnetothermally-triggered delivery
2.3.3 Magnetogenetics-based control
2.4 Acoustic control
2.4.1 Ultrasound-responsive delivery
2.4.2 Sonogenetics-mediated control
2.5 Other physical stimuli-responsive CRISPR systems
2.5.1 CRISPR-(d)Cas12
2.5.2 CRISPR-(d)Cas13
3. Comparisons among different physical trigger-induced strategies
3.1 Spatiotemporal precision
3.2 Tissue penetration
3.3 Reversibility
3.4 Off-target effect
3.5 Biocompatibility
4. Conclusion and outlook
図表リスト (リンク先はPubMed Central公開バージョンへ)
- Figure 1. Schematic illustration of sgRNA-guided Cas9 nuclease for DSBs, followed by endogenous repairing pathways: NHEJ or HDR pathways.
- Table 1. Advantages and disadvantages of chemical- and physical-inducible CRISPR-Cas9 control (overview).
- Figure 2. Illumination of physical stimuli-responsive CRISPR-Cas9 systems towards spatiotemporally precise control of genome engineering.
- Figure 3. Optical control of Cas9 for genome editing.
- Figure 4. Representative cases of optical control of dCas9 function for target gene activation or inhibition.
- Figure 5. Representative cases of optical control of nCas9 function for target gene activation or inhibition.
- Table 2 Chemical structures for light-responsive regulation of gRNA function.
- Figure 6. NIR light-controlled CRISPR-Cas9 delivery systems for gene editing in vivo.
- Figure 7. Illustration of the optogenetic regulation of genome editing mediated by the photoactivatable CRISPR-Cas9 nanosystem.
- Figure 8. Magnetic control strategies for CRISPR-Cas9 systems and their key characteristics.
- Figure 9. Physically tunable CRISPR (d)Cas12a systems.
- Figure 10.Physically tunable CRISPR (d)Cas13 systems.
- Table 3. Comparisons among different physical trigger-inducible CRISPR-Cas9 systems.
[出典] Review "Physical stimuli-responsive CRISPR-Cas9 systems for spatiotemporally precise control of genome engineering" Pan J, Li B [..] Sun SK. Theranostics. 2026-01-01. https://doi.org/10.7150/thno.122238 [所属] Tianjin Medical University (Tianjin Key Laboratory of Functional Imaging; Cell Biology) (中国), Fudan University (Zhongshan Hospital), Massachusetts General Hospital (Institute for Innovation in Imaging (i 3 )) (兼任: 米国)
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