CRISPR-Cas GEは、蛍光タンパク質、レポーター遺伝子、またはハイスループットシーケンス用のDNAバーコードの導入を介して、細胞系譜再構成の精度と解像度を大幅に向上させ、その応用範囲を拡大しました。中国医学科学院・北京協和医学院傘下医学実験動物研究所 (ILAS CAMS & PUMC)のShoulong Deng助教授を責任著者とするレビューは、細胞系統追跡におけるCRISPR-Cas9システムの応用例が取り上げられ [論文 Table 1参照]、中でも、従来のモデル動物（ゼブラフィッシュやマウスなど）、大型動物モデル（ブタ）、ヒト細胞またはオルガノイドにおける成功事例に焦点が当てられています。また、異種移植および再生医療における「移植した細胞の系統を追跡する」技術の活用と課題も取り上げられています。

[構成] 参考文献154件を含む15頁

• 1 INTRODUCTION
• 2 RESEARCH PROGRESS AND APPLICATION OF CELL LINEAGE TRACING
• 3 CRISPR-CAS9-MEDIATED CELL LINEAGE TRACING
• 3.1 CRISPR-Cas9-mediated cell lineage tracing techniques
• 3.2 CRISPR-Cas9-mediated cell lineage tracing information reading technique
• 3.3 Limitations and optimization of CRISPR-Cas9-mediated cell lineage tracing technique
• 4 CELL LINEAGE TRACING TECHNOLOGY
• 4.1 Application of cell lineage tracing technology in cultured cells
• 4.2 The application of cell lineage tracing technology in model animals
• 4.3 Application of cell lineage tracing technology in diseases
• 4.4 Application of cell lineage tracing technology in a mouse model
• 4.5 Application of cell lineage tracing technology in porcine model
• 4.6 Application of cell lineage tracing technology in nonhuman primate model
• 5 EVOLUTION OF CELL LINEAGE TRACING
• 6 FUTURE APPLICATIONS: ORGAN TRANSPLANTATION
• 7 SUMMARY

[図一覧]

• FIGURE 1 Lineage tracing via CRISPR-Cas9 coupled with single-cell sequencing. 　[右図に引用]
• FIGURE 2 The application of cell lineage tracing technology.
• FIGURE 3 The pig models for xenotransplantation and xenogeneic organ reconstruction.
• TABLE 1. Cell lineage tracing techniques：Direct microscopic observation (1983); Dye labelling (1986); Genetic markers (1999); CRISPR-Cas9-mediated fluorecent labeling (2013); CRISPR-Cas9 combined with Cre-LoxP system (2014); GESTALT (2016); Homing CRISPR barcodes (2017); SLENDR (2017); MEMOIR (2017); PiggyBac transposon system combined with CRISPR-Cas9 (2019)

[出典]

• "Advances in CRISPR-Cas9 in lineage tracing of model animals" Cao J, Guo Z, Xu X, Li P, Fang Y, Deng S. Animal Model Exp Med. 2025-06-10. https://doi.org/10.1002/ame2.70033 [所属] China Agricultural University, Institute of Laboratory Animal Sciences (ILAS)/Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Fujian Agriculture and Forestry University, Xianghu Laboratory, Jilin Agricultural University