細胞運命決定機構の解明には、細胞系譜の履歴と動的な表現型状態を結びつけることが不可欠です。シングルセルシーケンスとゲノム編集の発展により、細胞系譜追跡は観察研究から多次元的かつハイスループットな研究分野へと進化を遂げました。西湖大学で細胞系譜研究室を主催するShou-Wen Wang助教授と中国科学院上海生化学・生物学研究所のBin Zhou教授によるレビューでは、細胞系譜追跡の3つの主要な手法 （遺伝子マーカー依存；合成バーコード依存；内因性自然変異依存）を体系的に解説しています。[Figure 1引用下図参照]
さらに、マルチオミクス解析や空間プロファイリングとの統合、そして細胞系譜データから細胞運命を解読するための計算手法についても概説されています。各手法のトレードオフが詳細に説明され、実験設計のための体系的な指針が示され [Figure 5引用下図参照]、精密なクローン解析を臨床応用へと繋げるための新たなフロンティアも提示されています。
[構成] 参考文献151件を含む18頁
Introduction
Lineage tracing with genetic markers
Labeling cells with a single recombinase
Resolving complex contexts: Dual recombinase and logic gates
Dissecting heterogeneity: Clonal analysis with multicolor reporters
Practical considerations
Synthetic lineage tracing with engineered barcodes
Static barcoding
Evolvable barcoding
Practical challenges in synthetic lineage tracing
Natural lineage tracing with endogenous variants
Resolving histories via somatic DNA mutations
Resolving histories via mitochondrial DNA mutations
High-resolution lineage tracing via DNA methylation
Integrating lineage history with molecular and spatial states
Computational integration of lineage and state dynamics
Practical design guidance
Opportunities and future directions
[図表リスト]
- Figure 1 The three methodological pillars of lineage tracing for systematic cell fate analysis
- Figure 2 Genetic strategies for driver-induced lineage tracing and clonal analysis.
- Figure 3 Strategies, practical challenges, and readout modalities of synthetic barcoding.
- Figure 4 Natural lineage tracing using endogenous variants.
- Figure 5 A flowchart for selecting appropriate lineage tracing technologies.
- Table 1 Modern lineage tracing approaches.
- Table 2 Summary and evaluation of modern lineage tracing approaches.
[出典] REVIEW "Evolving strategies for lineage tracing: Genetic markers, synthetic barcodes, and natural variants" Zhixin Kang (1,8), Hui Chen (2,8), Siyang Li (3,8), Shou-Wen Wang (3,4,5,6), Bin Zhou (1,2,7). Cell Stem Cell. 2026-05-29. https://doi.org/10.1016/j.stem.2026.05.001
- Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China
- CAS CEMCS-CUHK Joint Laboratory, New Cornerstone Science Laboratory, Key Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China
- Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou 310024, Zhejiang, China
- School of Life Sciences, Westlake University, Hangzhou 310024, Zhejiang, China
- School of Science, Westlake University, Hangzhou 310024, Zhejiang, China
- Center for Interdisciplinary Studies, Westlake University, Hangzhou 310024, Zhejiang, China
- School of Life Science and Technology, ShanghaiTech University, Shanghai, China
- These authors contributed equally
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