Cas12a（発見時の呼称はCpf1）は、CRISPR-Cas9と同様に、RNAにガイドされて標的dsDNAに結合し切断する。Doudnaチームは今回、Lachnospiraceae bacterium由来Cas12a（LbCas12a）がdsDNA切断時に、ssDNAを非選択的に完全に分解するssDNase活性も備えていることを明らかにした。このssDNaseはヒト細胞内のDNAはほとんど二重螺旋構造をとっていることから、ヒトゲノム編集に与える影響は限定的である。一方で、研究チームはLbCas12a、crRNA、蛍光レポーターにリコンビナーゼポリメラーゼ増幅（RPA）を組み合わせることで、超高感度なDNA検出法"DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR)"を開発し、パピローマウイルス（HPV）のタイプ16と18のL1遺伝子超可変ループVを標的とするLbCas12a-crRNAにより、それぞれのタイプをアトモル濃度の感度で同定可能なことを示した。CRISP_SCIENCE@ScienceCrisp
Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA.
2018/02/19 10:44:53
&vquot;DETECTR&vquot;
#CRISPR-Cas12a target binding u… https://t.co/Me0DQ9KbgQ
Unexpected evolutionary benefit to phages imparted by bacterial #CRISPR-Cas9.
2018/02/19 10:44:00
Science Advances 0214.
https://t.co/BFQtLgt5mC
C8ORF37 is required for photoreceptor outer segment disc morphogenesis by maintaining outer segment membrane protei… https://t.co/U2DYdVmhlB
2018/02/19 10:43:19
Inner Workings: How the butterfly got its spots (and why it matters).
2018/02/19 10:41:44
PNAS. 0213.
&vquot;#CRISPR;researchers have discov… https://t.co/lk6vEnFwSM
PIM kinases are a potential prognostic biomarker and therapeutic target in neuroblastoma.
2018/02/19 10:39:28
Mol Cancer Ther 0213.
&vquot;Us… https://t.co/DMdbUysHDg
[Contradictory Results]Reverse transcriptase fused #CRISPR-Cas1 locus with RNA-seq expression necessitates revisiti… https://t.co/knaBZDCFrD
2018/02/19 10:38:42
The Application of NHEJ-#CRISPR/Cas9 and Cre-Lox System in the Generation of Bivalent Duck Enteritis Virus Vaccine… https://t.co/2pFy9Hr7KR
2018/02/19 10:38:29
#CRISPR Cas9 searches for a protospacer adjacent motif by one-dimensional diffusion.
2018/02/19 10:37:55
bioRxi 0213.
https://t.co/3dPt0vn32R
#CRISPR-Cas-Mediated Phage Resistance Enhances Horizontal Gene Transfer by Transduction.
2018/02/19 10:37:31
mBio 0213.
https://t.co/XtnCTMkvEZ
DNA mismatch repair and oligonucleotide end-protection promote base-pair substitution distal from a #CRISPR/Cas9-in… https://t.co/Y0WfvP9Qxx
2018/02/19 10:37:15
ssRNA/DNA-Sensors via Embedded Strand-Displacement Programs in #CRISPR/Cas9 Guides
2018/02/19 10:36:36
bioRxiv 0212.
https://t.co/gAHVya2kRz
Feasibility of a Conditional Knockout System for Chlamydia Based on #CRISPR Interference.
2018/02/19 10:35:51
Front Cell Infect Microbi… https://t.co/4D2FqSJL4u
#CRISPR/Cas9 generated human CD46, CD55 and CD59 knockout cell lines as a tool for complement research
2018/02/19 10:34:48
J Immunol Me… https://t.co/oCVvUvm8Pi
Monoclonal Cell Line Generation and #CRISPR/Cas9 Manipulation via Single-Cell Electroporation.
2018/02/19 10:33:52
Small 0212.
https://t.co/smU1Gkwn6V
Targeted knock-in of CreER T2 in zebrafish using #CRISPR/Cas9
2018/02/19 10:33:02
Cell Tissue Res 0212.
https://t.co/5iSKYrrTXt
New Developments in #CRISPR Technology: Improvements in Specificity and Efficiency.
2018/02/19 10:31:28
Curr Pharm Biotechnol 0209.
https://t.co/gZwZKXRONm
Genome-wide tracking of dCas9-methyltransferase footprints
2018/02/19 10:30:59
Nat Commun 0209.
#CRISPR
https://t.co/VzVOuRKAol
Unexpected heterogeneity derived from Cas9 ribonucleoprotein‐introduced clonal cells at the HPRT1 locus.
2018/02/19 10:29:21
Genes Cell… https://t.co/zmCZ8Tjro0
Primary Airway Epithelial Cell Gene Editing Using #CRISPR-Cas9.
2018/02/19 10:27:58
Methods Mol Biol 0209.
https://t.co/F248fImQre
Hallmarks of cancer: The #CRISPR generation.
2018/02/19 10:23:34
Eur J Cancer 0209
https://t.co/0Suo8tEgMo
Highly efficient #CRISPR gene editing in yeast enabled by double selection.
2018/02/19 10:23:20
bioRxiv 0209.
https://t.co/3jK60JoEr0
Conserved accessory proteins encoded with archaeal and bacterial Type III #CRISPR-Cas gene cassettes that may speci… https://t.co/1p23CMmGXN
2018/02/19 10:23:06
[Confirmatory Results]No unexpected #CRISPR-Cas9 off-target activity revealed by trio sequencingene-edited mice.
2018/02/19 10:22:50
bi… https://t.co/1ULX8mfDnq
An alternatively spliced form affecting the Marked Box domain of Drosophila E2F1 is required for proper cell cycle… https://t.co/UJxXtcIBwi
2018/02/19 10:16:02
Glucose repression can be alleviated by reducing glucose phosphorylation rate in Saccharomyces cerevisiae
2018/02/19 10:13:51
Sci Rep 0… https://t.co/pOMuZLdffB
Csde1 binds transcripts involved in protein homeostasis and controls their expression in an erythroid cell line.
2018/02/19 10:10:00
Sc… https://t.co/kRHJzWWqDF
Is #CRISPR an Ethical Game Changer?
2018/02/19 09:59:54
Schultz-Bergin M.
Journal of Agricultural and Environmental Ethics 0208.
https://t.co/SgO14D1oxb
#CRISPR/Cas9-Mediated Mosaic Mutation of SRY Gene Induces Hermaphroditism in Rabbits.
2018/02/19 09:59:12
Bioscience Reports 0208.
https://t.co/21kC9XZhcK
Heterologous and endogenous U6 snRNA promoters enable #CRISPR/Cas9 mediated genome editing in Aspergillus niger.
2018/02/19 09:58:58
Fu… https://t.co/M9fNXKs5nk
MELK expression correlates with tumor mitotic activity but is not required for cancer growth.
2018/02/19 09:58:27
eLife 0208.
&vquot;“generat… https://t.co/niSQyYZFtj
Long noncoding RNA PANDAR blocks CDKN1A gene transcription by competitive interaction with p53 protein in gastric c… https://t.co/XUp8aCapJl
2018/02/19 09:56:12
#CRISPR-Cas9, germinal cells and human embryo.
2018/02/19 09:50:30
Jouannet P.
Biol Aujourdhui (in French) 0207.
https://t.co/GydGYhdsIm
Generation of Hutat2: Fc knock-in primary human monocytes using #CRISPR/Cas9
2018/02/19 09:50:04
Mol Ther Nucleic Acids 0207.
https://t.co/jtq8QvflLm
Phosphorothioate-modified DNA oligonucleotides inactivate #CRISPR-Cpf1 mediated genome editing
2018/02/19 09:48:07
bioRxiv 0206.
https://t.co/OFfx2KfVgc
G-quadruplex motif at the 3'end of sgRNAs improves #CRISPR-Cas9 based genome editing efficiency.
2018/02/19 09:47:43
Chem Commun 0206.
https://t.co/T7boNvKzLe
Target identification of small molecules using large-scale #CRISPR-Cas mutagenesis scanning of essential genes
2018/02/19 09:47:26
Nat… https://t.co/Z4QkmPJCaM
Simplified footprint-free Cas9/#CRISPR editing of cardiac-associated genes in human pluripotent stem cells
2018/02/19 09:46:57
Stem Cel… https://t.co/UJHUdpYO3Q
#CRISPR interference-based specific and efficient gene inactivation in the brain.
2018/02/19 09:46:24
Nat Neurosci 0205
https://t.co/3j4xQGl6AO
Generation of human embryonic stem cell line with heterozygous RB1 deletion by #CRIPSR/Cas9 nickase
2018/02/19 09:46:02
Stem Cell Res 0… https://t.co/QzOTUZftUj
Could Failure in Preimplantation Genetic Diagnosis Justify Editing the Human Embryo Genome?
2018/02/19 09:41:23
Cell Stem Cell 0202… https://t.co/8HhPNLWpUv
Identifying Signalling Pathways Regulated by GPRC5B in β-Cells by #CRISPR-Cas9-Mediated Genome Editing.
2018/02/19 09:40:40
Cell Physio… https://t.co/xaoVtUTsVd
[FORWARD]#CRISPR-Cas Antiviral Strategies
2018/02/19 09:39:39
Jin DY, Berkhout B.
Virus Res 01/15
https://t.co/VE37TPF8aV
&vquot;This Special… https://t.co/GwTQEyEWc1
#CRISPR knock out of programmed cell death protein 1 enhances anti-tumor activity of cytotoxic T lymphocytes.
2018/02/19 09:39:03
Oncot… https://t.co/z2t4WdUbsE
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