[出典] "Ultrasensitive detection of gene-PIK3CA<H1047R> mutation based on cascaded strand displacement amplification and trans-cleavage ability of CRISPR/Cas12a" Deng Y, Cao G, Chen X, Yang M, Huo D, Hou C. Talanta. 2021-04-20. https://doi.org/10.1016/j.t ...
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タグ：リキッドバイオプシー
ctDNAに基づく診断実現に向けて, 標的DNA増幅が不要なCas12aバイオセンサーを開発
[出典] "CRISPR-Cas12a-Based Nucleic Acid Amplification-Free DNA Biosensor via Au Nanoparticle-Assisted Metal-Enhanced Fluorescence and Colorimetric Analysis" Choi JH, Lim J, Shin M, Paek SH, Choi JW. Nano Lett. 2020-12-21. https://doi.org/10.1021/acs.nano ...
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Cas9/sgRNAによる野生型DNA分解を介して血中循環腫瘍DNAの検出感度を1000倍向上
[出典] "Improved EGFR mutation detection sensitivity after enrichment by Cas9/sgRNA digestion and PCR amplification" Wang L [..] Hu Z, Luo D. Acta Biochim Biophys Sin (Shanghai) 2020-11-20. https://academic.oup.com/abbs/advance-article-abstract/doi/10.109 ...
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細胞外小胞プロテオーム解析から、ヒト癌の判定に有用な一連のバイオマーカーを同定
[出典] Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers. Hoshino A, Kim HS, Bojmar L, Gyan KE [..] Jarnagin WR, Lyden D. Cell 2020-08-20. https://doi.org/10.1016/j.cell.2020.07.009 Weill Cornell Medicine/東工大の星野歩子とDavi ...
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米国FDA, リキッドバイオプシーにNGSを組合せたコンパニオン診断検査法を初承認
[出典] FDA Approves First Liquid Biopsy Next-Generation Sequencing Companion Diagnostic Test. FDA NEWS RELEASE 2020-08-07. [注] CDx: companion diagnostic FDAは2020年8月7日に、ガーダントヘルス社のGuardant360を、致死性のEGFR T790M 遺伝子変異陽性転移非小 ...
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