[出典] "Metabolic engineering of Escherichia coli to enhance protein production by coupling ShCAST-based optimized transposon system and CRISPR interference" Chang CW [..] Hu YC. J Taiwan Inst Chem Eng. 2023-03-01. https://doi.org/10.1016/j.jtice.2023.10 ...
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タグ：代謝工学
CRISPRi-dCpf1によりackA遺伝子を下方制御しA. succinogenesにおけるコハク酸産生を亢進
[注] Cpf1 = Cas12a[出典] "Effects of down-regulation of ackA expression by CRISPR-dCpf1 on succinic acid production in Actinobacillus succinogenes " Chen C, Zheng P. AMB Express. 2023-01-26. https://doi.org/10.1186/s13568-023-01518-x [著者所属] https://d ...
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マイクロバイオームにおける代謝経路の多様性を利用して、関心のある代謝活性を示す微生物種に限定したdCas9活性誘導を実現
[出典] "Metabolically-targeted dCas9 expression in bacteria" Pellegrino GM [..] Edgell DR. Nucleic Acids Res 2-23-01-25. https://doi.org/10.1093/nar/gkac1248 [著者所属] Schulich School of Medicine and Dentistry, U Guelph ヒトの消化管（gastrointestinal: G ...
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ScrABBLE：CRISPR-dCas12aを用いた遺伝子回路カスケードによる多重化パスウエイの最適化
[注] ScrABBLE（Syntehtic crRNA Arrays Blend for Boosting and LEading）[出典] "CRISPR–dCas12a-mediated genetic circuit cascades for multiplexed pathway optimization" Wu Y [..] Liu L. Nat Chem Biol. 2023-01-16. https://doi.org/10.1038/s41589-022-01230-0 [ ...
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CRISPR-Cpf1 (Cas12a) を利用してD-パンテント酸を効率的に生産するコリネ菌を開発
[出典] "Enhanced production of D-pantothenic acid in Corynebacterium glutamicum using an efficient CRISPR–Cpf1 genome editing method" Su R [..] Peng H, Chen N, Li Y. Microb Cell Fact 2023-01-06. https://doi.org/10.1186/s12934-023-02017-1 [著者所 ...
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