[注] 5-ALA (5-アミノレブリン酸) https://ja.wikipedia.org/wiki/アミノレブリン酸[出典] "Modularized Engineering of Shewanella oneidensis MR-1 for Efficient and Directional Synthesis of 5-Aminolevulinic Acid" Jie Wu, Wu Jing [..] Dong-Feng Liu, Wen-Wei Li. M …
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タグ：代謝工学
分割型CRISPRを利用してC. viswanathiiにおける合理的なゲノム工学と代謝工学を実現
[出典] "Rational genome and metabolic engineering of Candida viswanathii by split CRISPR to produce hundred grams of dodecanedioic acid" Pham NN [..] Hu YC. Metab Eng 2023-03-20. https://doi.org/10.1016/j.ymben.2023.03.007[著者所属] National Tsing Hua U, …
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ShCASTをベースとする最適化トランスポゾンシステムとCRISPRiの組み合わせにより、大腸菌によるタンパク質生産を改善
[出典] "Metabolic engineering of Escherichia coli to enhance protein production by coupling ShCAST-based optimized transposon system and CRISPR interference" Chang CW [..] Hu YC. J Taiwan Inst Chem Eng. 2023-03-01. https://doi.org/10.1016/j.jtice.2023.10 …
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CRISPRi-dCpf1によりackA遺伝子を下方制御しA. succinogenesにおけるコハク酸産生を亢進
[注] Cpf1 = Cas12a[出典] "Effects of down-regulation of ackA expression by CRISPR-dCpf1 on succinic acid production in Actinobacillus succinogenes " Chen C, Zheng P. AMB Express. 2023-01-26. https://doi.org/10.1186/s13568-023-01518-x [著者所属] https://d …
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マイクロバイオームにおける代謝経路の多様性を利用して、関心のある代謝活性を示す微生物種に限定したdCas9活性誘導を実現
[出典] "Metabolically-targeted dCas9 expression in bacteria" Pellegrino GM [..] Edgell DR. Nucleic Acids Res 2-23-01-25. https://doi.org/10.1093/nar/gkac1248 [著者所属] Schulich School of Medicine and Dentistry, U Guelph ヒトの消化管（gastrointestinal: G …
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