[出典] "Exploring and engineering PAM-diverse Streptococci Cas9 for PAM-directed bifunctional and titratable gene control in bacteria" Wang J [..] Yan Y. Metab Eng 2022-10-29. https://doi.org/10.1016/j.ymben.2022.10.005 [著者所属] U Georgia, North Oconee …
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タグ:代謝工学
酵母ゲノム工学のためのCRISPR-Casシステムのアセンブリーとin vivo編集を、最小限の試薬と時間で実現するACtivE法を開発
[出典] "ACtivE: Assembly and CRISPR-Targeted in Vivo Editing for Yeast Genome Engineering Using Minimum Reagents and Time" Malcı K [..] Rios-Solis L. ACS Synth Biol 2022-10-17. https://doi.org/10.1021/acssynbio.2c00175 [著者所属] U Edinburgh, Newcastle …
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T7ポリメラーゼとCRISPR-dCas9を組み合わせて酵母で機能するモジュール型ブーリアン論理ゲート構築法
[出典] "Modular, Synthetic Boolean Logic Gates Enabled in Saccharomyces cerevisiae through T7 Polymerases/CRISPR dCas9 Designs" Presnell KV, Melhem O, Morse NJ, Alper HS. ACS Synth Biol 2022-10-07. https://doi.org/10.1021/acssynbio.2c00327 [著者所属] Univ …
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グローバルレギュレーターmcrAを標的とするin vitro生成CRISPR-Cas9 RNPにより、Aspergillus wentiiの二次代謝産物BGCを覚醒
[注] BCG (biosynthetic gene clusters/生合成遺伝子群)[出典] "Manipulation of the Global Regulator mcrA Upregulates Secondary Metabolite Production in Aspergillus wentii Using CRISPR-Cas9 with In Vitro Assembled Ribonucleoproteins" Yuan B [..] Wang CCC. AC …
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高温耐性酵母K. marxianusの効率的代謝工学を実現するRNAポリメラーゼII駆動CRISPR-Cas9システム
[出典] "RNA polymerase II-driven CRISPR-Cas9 system for efficient non-growth-biased metabolic engineering of Kluyveromyces marxianus " Bever D, Wheeldon I, da Silva N. Metab Eng Commun 2022-09-24. https://doi.org/10.1016/j.mec.2022.e00208 [著者所属] Uni …
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