CRISPR/Cas9技術とBioIDをベースとする近接ラベリング技術の融合[出典] PROTOCOL "In vivo Characterization of Endogenous Protein Interactomes in Drosophila Larval Brain, Using a CRISPR/Cas9-based Strategy and BioID-based Proximity Labelling" Uçkun E [..] P ...
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タグ：分子間相互作用
[プロトコル] Cas9を介したDeep Mutattional Scanningにより，内在遺伝子座の点変異が，細胞内PPIに及ぼす影響を探る法
[注] PPI: タンパク質間相互作用[出典] PROTOCOL "Deep Mutational Scanning of Protein–Protein Interactions Between Partners Expressed from Their Endogenous Loci In Vivo" Dubé AK, Dandage R, Dibyachintan S, Dionne U, Després PC, Landry CR. In: Devaux F. (e ...
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タンパク質の１残基変異が引き起こすがんに特異的なタンパク質間相互作用'neoPPI'の包括的同定
[注] neoPPI: neo-protein-protein interaction[出典] "Systematic discovery of mutation-directed neo-protein-protein interactions in cancer" Mo X, Niu Q, Ivanov AA [..] Fu H. Cell 2022-05-04. https://doi.org/10.1016/j.cell.2022.04.014 [著者所属] Emory U Sc ...
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タンパク質のアミノ酸残基に発生する1重/2重変異それぞれが，安定性と結合親和性に及ぼす影響を，自由エネルギーのランドスケープとして俯瞰
[出典] "Mapping the energetic and allosteric landscapes of protein binding domains" Faure AJ, Domingo J, Schmiedel JM [..] Lehner B. Nature 2022-04-06. https://doi.org/10.1038/s41586-022-04586-4; [NEWS AND VIEWS] "Democratizing the mapping of gene mutatio ...
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CRISPR-Cas12aをベースとするPPI検出プラットフォーム'Cas-PPlor'
[出典] "P53-MDM2 interaction monitoring and inhibitors potency evaluation based on CRISPR-Cas12a sensing platform" Zhu M, Pan J [..] Su G, Yu Y. Sens Actuators B Chem 2022-03-12. https://doi.org/10.1016/j.snb.2022.131710 南通大学に南京大学が加わった研究 ...
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