blogging about crisp bio-S&T, especially CRISPR.


National Center of Gerontology (北京)からの論文[出典] "Detection of Epstein-Barr virus encoded RNA in fixed cells and tissues using CRISPR/Cas-mediated RCasFISH" Chen K, Wang M, Zhang R, Li J. Anal Biochem. 2021-04-26. ... もっと読む

[出典] "CRISPR-Sunspot: Imaging of endogenous low-abundance RNA at the single-molecule level in live cells" Sun NH, Chen DY, Ye LP, Sheng G, Gong JJ, Chen BH, Lu YM, Han F. Theranostics 2020-09-02. [注] CRISPR-Sunspotは"Suntag-mediated single molecule RN ... もっと読む

2020-09-16 PepCBとPepTag系の図解として、Figure 2 - b の引用に日本語注を付けた図を追加2020-09-14 初稿[出典] "A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies" Traenkle B., Segan S., Fagbadebo FO, Kaiser PD, R ... もっと読む

[出典] "Dynamic High-Sensitivity Quantitation of Procollagen-I by Endogenous CRISPR-Cas9 NanoLuciferase Tagging" Calverley BC, Kadler KE, Pickard A. Cells. 2020-09-10.  University of Manchesterの研究チームは今回、DyProQ (dynamic protein quantitation)と ... もっと読む

[リンクと図のみ] "Background-suppressed live visualization of genomic loci with an improved CRISPR system based on a split fluorophore" Chaudhary N [..] Kim H. Genome Res 2020-09-04.  Figure 1. SunTag split-sfGFP CRISPR system enables background suppress ... もっと読む