[出典] "Visualizing the Nucleome Using the CRISPR–Cas9 System: From in vitro to in vivo " Maloshenok LG [..] Zherdeva VV. Biochemistry (Moscow) 2022-02-20. https://doi.org/10.1134/S0006297923140080 [著者所属] Bach Institute of Biochemistry (Moscow), ...
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タグ：可視化
PERとCRISPR/dCas9の組み合わせによる低反復性遺伝子座のin situ 可視化
[注] PER（primer-exchange reaction/プライマー交換反応）[出典] "CasSABER for Programmable In Situ Visualization of Low and Nonrepetitive Gene Loci" Li Y, Huang D, Pei Y [..] Zhang J, Hou H, Zhang K, Li J. Anal Chem 2023-01-26. https://doi.org/10.1021/acs ...
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[プロトコル] CRISPR-Cas9 RNPによりiPS細胞における効率的な両アレルタギング
[出典] PROTOCOL "Efficient bi-allelic tagging in human induced pluripotent stem cells using CRISPR" Ren X, Takagi MA, Shen Y. STAR Protoc 2023-02-01. https://doi.org/10.1016/j.xpro.2023.102084 [著者所属] UCSF. UCSFの研究チームが、ヒトiPS細胞におけるタン ...
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dCas13を利用して、ゼブラフィッシュ胚発生中の内在mRNAの可視化と追跡を実現
[出典] "CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryos" Huang Y [..] Chen L-L. Genome Biol. 2023-01-19. https://doi.org/10.1186/s13059-023-02848-6 [著者所属] Shanghai Institute of Biochemistry ...
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sgRNAを蛍光標識することで、CRISPR-dC13システムによるライブセルRNAイメージングを実現
[出典] "Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers" Tang H [..] Weng X, Zhou X. Chem Sci 2022-11-08. https://doi.org/10.1039/d2sc04656c [著者所属] Wuhan U, ShanghaiTech U RNAが様々な ...
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