blogging about crisp bio-S&T, especially CRISPR.


University of Regensburgの研究グループからの報告.[出典] "Decoupling the bridge helix of Cas12a results in a reduced trimming activity, increased mismatch sensitivity and impaired conformational transitions" Wörle E, Jakob L, Schmidbauer A, Zinner G, Gro ... もっと読む

[出典] "gEL DNA: A Cloning- and Polymerase Chain Reaction–Free Method for CRISPR-Based Multiplexed Genome Editing" Randazzo P, Bennis NX, Daran J-M, Daran-Lapujade P. CRISPR J. 2021-04-23.[注] gELは,"gRNA Express ... もっと読む

[出典] "Switching the activity of Cas12a using guide RNA strand displacement circuits" Oesinghaus L, Simmel FC. Nat Commun. 2019-05-07. Technical University Munichの研究チームの報告遺伝子の編集と制御に加えて核酸 ... もっと読む

[出典] "A novel colorimetric aptasensor for ultrasensitive detection of aflatoxin M1 based on the combination of CRISPR-Cas12a, rolling circle amplification and catalytic activity of gold nanoparticles" Abnous K [..] Taghdisi SM. Analytica Chimica Acta. 2 ... もっと読む

[出典] "CRISPR/Cas12a-based on-site diagnostics of Cryptosporidium parvum IId-subtype-family from human and cattle fecal samples" Yu F [..] Zhang L.  Parasites Vectors. 2021-04-02. 河南農業大学の研究グループの ... もっと読む