crisp_bio

crisp_bioはCRISPRのオーラを浴びる中で思いついたブログ名でしたが,2021年からは専ら,CRISPR技術の開発と応用ならびにSRAS-CoV-2/COVID-19に関する投稿になってしまいました.データベース,生物資源センター,マイクロバイオーム,タンパク質デザインを取り上げたいと思いつつ.

タグ:Cas12a

[出典] "Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR Technology" Pang B [..] Zhang H, Le C. Anal Chem 2020-11-26. https://doi.org/10.1021/acs.analchem.0c04 ... もっと読む

In utero Perturb-Seq, ASD risk genes; conditional KO, Leishmania RAD51 & paralogues; temperature-tolerant LbCas9 for plant; shoot apical meristem domain-specific CRISPR ... もっと読む

[出典] "Digital CRISPR/Cas-Assisted Assay for Rapid and Sensitive Detection of SARS-CoV-2" Park JS, Hsieh K, Chen L, Kaushik A, Trick AY, Wang TH. Adv Sci. 2020-11-20. https://doi.org/10.1002/advs.202003564[注] deCOViD: digitization‐enhanced CRISPR/Cas‐ ... もっと読む

[出典] "CRISPAltRations: a validated cloud-based approach for interrogation of double-strand break repair mediated by CRISPR genome editing" Kurgan G [..] McNeill MS. bioRxiv. 2020-11-15. [Preprint] ; CRISPAlt-Rations Prototype Webサイト https://idtcrispr ... もっと読む

[出典] "Highly efficient and safe genome editing by CRISPR-Cas12a using CRISPR RNA with a ribosyl-2′-O-methylated uridinylate-rich 3′-overhang in mouse zygotes" Ha DI, Lee JM [..] Kim YS. Exp Mol Med. 2020-11-09.  KRIBB, Korea University of Science and ... もっと読む

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