[出典] "CRISPR-mediated protein-tagging signal amplification systems for efficient transcriptional activation and repression in Saccharomyces cerevisiae " Zhai H, Ci L, Xiong Z, Qi Q, Hou J. Nucleic Acids Res 2022-05-31. https://doi.org/10.1093/nar/gkac4 ...
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タグ:Cas12a
"RESET"効果:標的一本鎖DNAの3’末端の伸長を介してCas12aのトランス切断活性を向上
[出典] "RESET" Effect: Random Extending Sequences Enhance the Trans-Cleavage Activity of CRISPR/Cas12a" Ma JY [..] Wang J, Kong DM. Anal Chem. 2022-05-26. https://doi.org/10.1021/acs.analchem.2c01401 [著者所属] Nankai U (天津), Tianjin Medical U;グラフ ...
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Cas12aをベースとする電気化学的バイオセンサーによるcTnIの高感度検出を介した心血管疾患の早期診断
[注] cTnI: 心筋トロポニンI[出典] "CRISPR/Cas12a-based electrochemical biosensor for highly sensitive detection of cTnI" Chen H, Li Z [..] Chen Q, Wu L. Bioelectrochemistry 2022-05-19. https://doi.org/10.1016/j.bioelechem.2022.108167 [著者所属] 湖北医薬 ...
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CRISPR/Cpf1と3′末端を最大限切断したcrRNAにより微生物ゲノムの効率的1塩基編集を実現
[出典] "Efficient Single-Nucleotide Microbial Genome Editing Achieved Using CRISPR/Cpf1 with Maximally 3′-End-Truncated crRNAs" Lee HJ [..] Lee SJ. ACS Synth Biol. 2022-05-18. https://doi.org/10.1021/acssynbio.2c00054 [著者所属] Chung-Ang University, KRI ...
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CRISPR/Cas12aトランス切断活性を各種DNA修飾酵素により制御する
[出典] "Modulation of CRISPR/Cas12a trans -cleavage activity by various DNA-modifying enzymes" Han J, Park JS [..] Park KS. Microhemical J. 2020-05-20. https://doi.org/10.1016/j.microc.2022.107606 [著者所属] Konkuk University (Seoul) 近年,Cas12aの標的 ...
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